Barium in PDB 4ria: FAN1 Nuclease Bound to 5' Phosphorylated Nicked Dna

All present enzymatic activity of FAN1 Nuclease Bound to 5' Phosphorylated Nicked Dna:
3.1.4.1;

The structure of FAN1 Nuclease Bound to 5' Phosphorylated Nicked Dna, PDB code: 4ria was solved by N.P.Pavletich, R.Wang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	40.00 / 3.00
Space group	P 2 21 21
Cell size a, b, c (Å), α, β, γ (°)	87.813, 100.317, 212.465, 90.00, 90.00, 90.00
R / Rfree (%)	22.4 / 26.7

The binding sites of Barium atom in the FAN1 Nuclease Bound to 5' Phosphorylated Nicked Dna (pdb code 4ria). This binding sites where shown within 5.0 Angstroms radius around Barium atom.
In total 2 binding sites of Barium where determined in the FAN1 Nuclease Bound to 5' Phosphorylated Nicked Dna, PDB code: 4ria:
Jump to Barium binding site number: 1; 2;

Barium binding site 1 out of 2 in the FAN1 Nuclease Bound to 5' Phosphorylated Nicked Dna


Mono view


Stereo pair view

A full contact list of Barium with other atoms in the Ba binding site number 1 of FAN1 Nuclease Bound to 5' Phosphorylated Nicked Dna within 5.0Å range: probe atom residue distance (Å) B Occ A:Ba1101 b:54.2 occ:1.00 OD2 A:ASP960 2.9 52.9 1.0 O A:VAL976 3.0 52.5 1.0 OE2 A:GLU975 3.0 52.0 1.0 OD1 A:ASP960 3.0 51.8 1.0 CG A:ASP960 3.3 51.6 1.0 OD2 A:ASP981 3.6 65.1 1.0 CD A:GLU975 3.7 51.3 1.0 OE1 A:GLU975 3.7 52.9 1.0 OD1 A:ASP981 3.8 60.2 1.0 CG A:ASP981 4.1 63.9 1.0 C A:VAL976 4.1 51.7 1.0 NZ A:LYS977 4.2 57.2 1.0 OE2 A:GLU815 4.4 69.0 1.0 NE2 A:GLN987 4.4 34.9 1.0 N A:VAL976 4.6 49.3 1.0 OP1 F:DC3 4.6 71.9 1.0 CA A:VAL976 4.8 51.1 1.0 CB A:ASP960 4.8 49.3 1.0 CB A:VAL976 4.9 51.1 1.0 CD A:LYS977 4.9 52.9 1.0

Barium binding site 2 out of 2 in the FAN1 Nuclease Bound to 5' Phosphorylated Nicked Dna


Mono view


Stereo pair view

A full contact list of Barium with other atoms in the Ba binding site number 2 of FAN1 Nuclease Bound to 5' Phosphorylated Nicked Dna within 5.0Å range: probe atom residue distance (Å) B Occ B:Ba1101 b:61.9 occ:1.00 OD2 B:ASP960 2.9 56.6 1.0 OE2 B:GLU975 3.0 53.7 1.0 O B:VAL976 3.0 58.8 1.0 OD1 B:ASP960 3.0 57.3 1.0 CG B:ASP960 3.3 55.0 1.0 OE1 B:GLU975 3.6 51.1 1.0 OD2 B:ASP981 3.6 69.1 1.0 CD B:GLU975 3.6 51.9 1.0 OD1 B:ASP981 3.9 70.4 1.0 CG B:ASP981 4.1 69.4 1.0 C B:VAL976 4.1 55.1 1.0 NZ B:LYS977 4.3 54.0 1.0 OE2 B:GLU815 4.3 74.1 1.0 NE2 B:GLN987 4.5 36.4 1.0 N B:VAL976 4.6 51.3 1.0 CA B:VAL976 4.8 52.7 1.0 OP1 L:DC3 4.8 58.8 1.0 CB B:ASP960 4.8 52.5 1.0 CB B:VAL976 4.9 51.3 1.0

R.Wang, N.S.Persky, B.Yoo, O.Ouerfelli, A.Smogorzewska, S.J.Elledge, N.P.Pavletich. Dna Repair. Mechanism of Dna Interstrand Cross-Link Processing By Repair Nuclease FAN1. Science V. 346 1127 2014.
ISSN: ISSN 0036-8075
PubMed: 25430771
DOI: 10.1126/SCIENCE.1258973